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Experal

Medical

Antibody Engineering

Sold byBooks for Life

SKU: 9780199635931

RM168.68

Only 10 left in stock

Description

Recombinant DNA techniques have revolutionized the isolation and production of antibodies. This volume describes methods and technologies which will allow the researcher to isolate a new antibody, analyse its properties, format the correct antibody or fragment, and produce sufficient quantities for experimental use. Topics in this volumes include: generation and analysis of antibodies; antibody repertoires; antibody screening and selection; measuring antibody affinities; sequence analysis; antibody engineering and production; conversion of rodent antibodies to human antibodies by CDR grafting or guided selection; choosing and optimizing effector functions; preparation and use of antibody-based molecules in eukaryotic and prokaryotic systems; scaling up manufacture; and generation of high-affinity high-specificity human antibodies in appropriate formats. Antibody Engineering is a unique manual of recombinant DNA methods for all those working with antibodies in research, diagnosis, and therapeutics. Each chapter is written by a leading researcher in the field and provides essential background information, fully tested protocols, sample data from using these methods, trouble-shooting comments, and key hints and tips for success. This book is intended for post-graduates, post-doctoral researchers, and other academic staff involved in both clinical and molecular biological applications of antibodies.Table of Content1: Construction and use of antibody gene repertoires. 2: Affinity maturation of antibodies using phage display. 3: Human antibody repertoires in transgenic mice: manupulation and transfer of YACs. 4: Measuring antibody affinities in solution. 5: Measuring antibody affinities using Bio-sensors. 6: Analysis of human antibody sequences. 7: Rodent to human antibodies by CDR grafting. 8: Rodent to human antibodies by phage display. 9: Choosing and manipulating effector functions. 10: Secretion and purification of antibody fragments expressed in bacteria. 11: Production of single chain Fv monomers and multimers. 12: Expression of immunoglobulin genes in mammalian cells. 13: In vitro linkage of antibody fragments into dimeric and multimeric forms

Specifications

ISBN-13: 9780199635931
Author: Chiswell David J.
Publisher: Oxford University Press
Publication Date: 1996-06-01
Binding: hardcover
Condition: new
Pages: 325
Language: english
Country of Origin: United States
Weight (g): 400
Height (mm): 30
Length (mm): 198
Width (mm): 132

Frequently Asked Questions

: Standard shipping to Malaysia typically takes 1–2 business days after dispatch. Express options are available at checkout.
: We offer a 30-day return window for most products. Items must be unused, in their original packaging, and accompanied by proof of purchase to qualify for a full refund.
: We accept all major credit and debit cards (Visa, Mastercard, American Express), as well as Apple Pay and Google Pay. All transactions are processed securely via Stripe and charged in MYR.
: Yes — we never store your card details. All payments are handled by Stripe, a PCI-DSS Level 1 certified payment processor, using industry-standard TLS encryption.
: Some products can be backordered — you will see a note on the product page if this is the case. We will ship as soon as stock arrives and keep you updated via email.
: Once your order is dispatched you will receive a shipping confirmation email containing your tracking number and a link to track your parcel in real time.