Dispersity, Structure and Phase Changes of Proteins and Bio Agglomerates in Biotechnological Processes

This book serves as a comprehensive summary of the priority program SPP 1934, which focused on understanding the dispersity, structure, and phase changes of proteins and bio-agglomerates in biotechnological processes. Through contributions from various research groups, the program explored how sensitive proteins and bio-agglomerates are affected by the process environment during fermentation, downstream processing, and formulation. It investigated these effects across three size scales: microscale, encompassing single proteins, clusters, crystals, and virus-like particles; mesoscale, focusing on cells and cell clusters; and macroscale, examining overall process dynamics. The main objective was to enhance biotechnological process chains by elucidating the mechanical, thermal, and chemical stresses that impact protein and bio-agglomerate structures. By gaining insights into these stressors, the program aimed to enable precise control measures to mitigate denaturation and unfavorable growth of proteins and cells. This compilation seeks to contribute to the optimization of biotechnological processes, facilitating advancements in various industries. Table of Content1. Correlation of structure and properties of enzyme crystals in consideration of downstream processing and formulation.- 2. Multiscale Model-Based Investigation of Functional Macromolecular Agglomerates for Biotechnological Applications.- 3. Modeling-based Monitoring and Control of Protein Crystallization in Bioprocesses.- 4. Changes in structure, dispersity and phase behavior of proteins: The virus-like particles in the presence of nucleic acids.Biographical NoteProf. Dr.-Ing. Arno Kwade is a professor of Mechanical Process Engineering / Particle Technology at the Technische Universität Braunschweig according to the website. He is the head of the Institute of Particle Technology. Prof. Kwade's research interests include stirred media mills, comminution, and battery technology. He has written numerous publications on these topics. Prof. Kwade is also involved in several professional organizations including the European Federation of Chemical Engineering and the BMBF Advisory Board Battery Research Germany. Dr.-Ing. Ingo Kampen is a researcher at the Institute for Particle Technology (iPAT) at the Technische Universität Braunschweig. His research area is pharmaceutical and bioparticle technology. He currently leads the research group "Bio- and Pharmapartikeltechnik". Dr. Kampen teaches several courses including "Fundamentals of Environmental Protection Engineering", "Mechanical Process Engineering 1 for Biotechnologists", "Microscopy and Particle Measurement in the Micro and Nanometer Range", and "Mechanical Process Engineering 2 for Biotechnologists".This book serves as a comprehensive summary of the priority program SPP 1934, which focused on understanding the dispersity, structure, and phase changes of proteins and bio-agglomerates in biotechnological processes. Through contributions from various research groups, the program explored how sensitive proteins and bio-agglomerates are affected by the process environment during fermentation, downstream processing, and formulation. It investigated these effects across three size scales: microscale, encompassing single proteins, clusters, crystals, and virus-like particles; mesoscale, focusing on cells and cell clusters; and macroscale, examining overall process dynamics. The main objective was to enhance biotechnological process chains by elucidating the mechanical, thermal, and chemical stresses that impact protein and bio-agglomerate structures. By gaining insights into these stressors, the program aimed to enable precise control measures to mitigate denaturation and unfavorable growth of proteins and cells. This compilation seeks to contribute to the optimization of biotechnological processes, facilitating advancements in various industries.Presents tailored approaches for various biotechnological tasks, fostering efficiency in the field Provides an overview of the program SPP 1934, elucidating main findings of all participating workgroups Contributes to the optimization of various Biotechnological Processes