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Experal

Science

Lipid Analysis

Sold byBooks for Life

SKU: 9780199630981

$70.00

Only 10 left in stock

Description

This book presents a practical guide to the laboratory techniques commonly used in the analysis of lipids. It covers methods of lipid extraction in solvent and solid phase systems, as well as methods of derivation formation needed for a number of analytical procedures such as gas liquid chromatography and mass spectrometry. The book also describes in practical detail the techniques for complete analysis of both isolated lipids and lipid mixtures. These include GLC, HPLC, TLC and NMR. These molecules are found in every living system, and are of interest to the chemical, biotechnological and pharmaceutical industries in addition to academic research workers.Table of ContentPart 1 Introduction: fatty acids; other monofunctional compounds; lipids; acylglycerols (glycerides); phospholipids; waxes; ether lipids; glycosyldiacylglycerols; sterol esters; sphingolipids; Frank D. Gunstone. Part 2 Extraction of lipids and derivative formation: preliminary treatment of tissues; treatment of samples; derivative formation; isolation (extraction) of volatiles; Shiela Hamilton, et al. Part 3 Thin-layer chromatography: materials and apparatus; application of sample and development of plates; TLC on standard silica; TLC on modified silica; detection systems; quantitation; TLC on coated quartz micro-columns; R. James Henderson and Douglas R. Tocher. Part 4 Gas chromatography of lipids: applications of GC analysis of lipids; basic principles; basic instrumentation; GC column detection; compound identification; applications; trouble-shooting; Richard P. Evershed. Part 5 High-performance liquid chromatography: theory of HPLC; mechanisms of separation; equipment; fractionation into lipid classes; separation of lipid classes; separation of fatty acids; separation of glycerolipids; separation of phospholipids; separation of cholesterol and cholesterol esters; Peter A. Sewell. Part 6 Radiotracers in lipid analysis: measurement of labelled compounds; radiochromatography; radioisotope dilution analysis and radioimmunoassay; radiochemical purity; availability of radiolipids and related compounds; health and safety; Colin G. Taylor. Part 7 High-resolution 1H and 13C NMR: 1H NMR spectroscopy; 13C NMR spectroscopy; Frank D. Gunstone. Part 8 Mass spectroscopy of lipids: basic principles; basic instrumentation; applications; Richard P. Evershed. Appendix: suppliers of specialist items

Specifications

ISBN-13: 9780199630981
Author: Hamilton Shiela
Publisher: Oxford University Press
Publication Date: 1992-12-01
Binding: hardcover
Condition: new
Pages: 329
Language: english
Country of Origin: United States
Weight (g): 400
Height (mm): 30
Length (mm): 198
Width (mm): 132

Frequently Asked Questions

: Standard shipping to United States typically takes 1–2 business days after dispatch. Express options are available at checkout.
: We offer a 30-day return window for most products. Items must be unused, in their original packaging, and accompanied by proof of purchase to qualify for a full refund.
: We accept all major credit and debit cards (Visa, Mastercard, American Express), as well as Apple Pay and Google Pay. All transactions are processed securely via Stripe and charged in USD.
: Yes — we never store your card details. All payments are handled by Stripe, a PCI-DSS Level 1 certified payment processor, using industry-standard TLS encryption.
: Some products can be backordered — you will see a note on the product page if this is the case. We will ship as soon as stock arrives and keep you updated via email.
: Once your order is dispatched you will receive a shipping confirmation email containing your tracking number and a link to track your parcel in real time.