Transcription Factors

Since the publication of the first edition, a wide range of new methodologies have been developed to facilitate studies on both isolated parts of the genome and the genome as a whole. This edition has been updated to provide a comprehensive guide to the methods available to characterize the function and activity of an individual transcription factor. All the original chapters have been fully updated or rewritten and additional chapters cover the use of in vitro transcription assays, analysis of chromatin structure, use of the genomic binding site assay and analysis of transcription factor modifications. As with the previous edition, the book starts with a series of chapters concerned with characterizing the proteins binding to a specific DNA sequence and then a chapter on more detailed characterization of the protein itself. The next two chapters describe the isolation of cDNA clones encoding a transcription factor using oligonucleotides predicted from protein sequence and screening of a cDNA expression library.Table of Content1: The DNA mobility shift assay; 2: Footprint analysis of DNA-protein complexes in vitro and in vivo ; 3: In vitro transcription and characterization of transcription factors; 4: Purification and cloning of DNA binding transcription factors; 5: Cloning transcription factors from a cDNA expression library; 6: Cloning transcription factors by sequence similarity; 7: Identification of target genes for a transcription factor by genomic binding site cloning; 8: Analysis of cloned factors; 9: Neuronal promoter analysis by in vitro transcription using nuclear extracts from rat brain; 10: Preparation of chromatin templates for transcription studies; 11: Analysis of transcription factor modifications; Appendix: Suppliers of specialist items.